OriDB - ARS432.5

Status: Confirmed ARS: Confirmed by ARS assay and identified by 9 genome-wide studies.

Genomic Location: Chr4:1165998-1166221
Within tandem intergenic space between YDR345C and YDR346C.
• View at UCSC genome browser
• View on SGD Chromosome Features Map
• View at SGD gbrowser site
• View at Ensembl browser

DNA Sequence:

Origin Sequence Elements: ACS confirmed.

Helical Stability: Minimum -145.5 ΔG° (kcal/mol) at location 1166178.

Time of Origin Replication (Trep): Raghuraman et al. (2001) — Trep: 12.6 min.
Yabuki et al. (2002) — Trep: 17.0 min.
Alvino et al. (2007) — Peak first observed at 10.0 min.

Origin activity in HU: Origin activity detected in HU — more details.

ARS at IV-1166 has unique ID: 144

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ARS at IV-1166 has unique ID: 144

Studies that cloned this origin

Nieduszynski et al. (2006): Chr4:1165998-1166221

Studies that analyzed this origin by 2D gel

None curated.

Studies that detected this origin by chromatin immunoprecipitation (ChIP)

Xu et al. (2006): Chr4:1165550-1166850

Shor et al. (2009): Chr4:1165700-1166500 (orc2-1/wt peak ratio: 0.44)

Szilard et al. (2010): Chr4:1166165-1166175

MÃ¼ller et al. (2010): Chr4:1165700-1166975 (orc1-bah-delta/wt peak ratio: 0.29)

Studies that measured the replication time of this origin

Raghuraman et al. (2001): Chr4:1164550 (Trep: 12.6 min.) (Confidence: 9)

Yabuki et al. (2002): Chr4:1162291 (Trep: 17.0 min.)

Alvino et al. (2007): Chr4:1162400 (Peak first observed at 10.0 min.)

Studies that measured the activity of this origin in hydroxyurea (HU)

Feng et al. (2006): Chr4:1167000 (Activity detected in: wild-type, rad53)

CrabbÃ© et al. (2010): Chr4:1165998-1166221 (Activity detected in: wild-type, rad9, rev3 rad30, eco1, ctf4, ddc1, rad24, pol2-12, elg1, tof1, mrc1-AQ, mrc1-AQ rad9, ctf8, ctf18, mrc1, dcc1, ctf18 rad9, mec1-100, rad53, mec1)

Studies that predicted the location of this origin

This origin has not been predicted by any curated study.

ARS at IV-1166 has unique ID: 144

Studies that confirmed an essential ACS element

Nieduszynski et al. (2006):

T

A

T

A

C

A

T

G

T

Studies that predicted an essential ACS element

Xu et al. (2006):

A

T

A

T

A

T

A

C

A

T

G

T

C

G

A

T

A

T

A

T

C

T

Eaton et al. (2010):

T

A

T

A

C

A

T

G

T

C

G

A

T

A

T

A

T

C

T

C

ACS LOGO:

ARS at IV-1166 has unique ID: 144

Alignments from the UCSC genome browser

Confirmed ACS

S. cerevisiae

T

C

G

T

A

C

A

C

T

A

G

C

A

T

A

T

A

T

A

C

A

T

G

T

C

G

A

T

A

T

A

T

C

T

C

T

C

T

S. kudriavzevii

A

T

G

T

G

A

C

T

G

T

C

A

C

A

T

A

T

A

C

A

T

G

T

C

G

A

T

A

T

G

T

C

T

A

C

A

S. mikatae

C

A

T

G

A

G

C

T

C

T

A

C

A

T

A

T

A

T

G

T

A

C

G

T

G

T

C

T

-

G

T

A

T

G

T

G

C

A

S. paradoxus

T

C

T

A

G

C

T

A

T

A

G

C

A

T

A

T

A

T

A

C

A

T

G

T

C

A

G

T

A

T

A

T

A

T

C

T

C

G

S. bayanus

T

G

T

G

C

T

A

C

T

G

T

A

T

A

T

A

T

A

C

A

T

G

T

C

A

G

A

T

G

T

G

T

C

T

A

G

*

:

*

:

*

:

*

:

*

:

*

:

*

:

*

:

*

:

*

:

*

:

*

:

ARS at IV-1166 has unique ID: 144

These notes are manually curated. To submit notes for this replication origin site please contact us.

Friday, 24th March 2006 - Anne Donaldson:

ARSIV-1166 lies 7kb from another origin, ARSIV-1159. Wyrick et al (2001) identified ARSIV-1159 (as proARS432), but did not identify ARSIV-1166 (which gave some ChIP signal but below thresholds to be assigned as an origin).

ARS at IV-1166 has unique ID: 144