OriDB - ARS at III-224

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Status: Dubious ARS: Identified by 10 genome-wide studies.

Genomic Location: Chr3:222963-224792
Resolution insufficient to assign intergenic space.
• View at UCSC genome browser
• View on SGD Chromosome Features Map
• View at SGD gbrowser site
• View at Ensembl browser

DNA Sequence:

Origin Sequence Elements: No elements have been reported for this origin.

Helical Stability: Minimum -172.6 ΔG° (kcal/mol) at location 223523.

Time of Origin Replication (Trep): Raghuraman et al. (2001) — Trep: 14.8 min.
Yabuki et al. (2002) — Trep: 24.2 min.
Alvino et al. (2007) — Peak first observed at 10.0 min.

Origin activity in HU: Origin activity detected in HU — more details.

ARS at III-224 has unique ID: 84

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ARS at III-224 has unique ID: 84

Studies that cloned this origin

None curated.

Studies that analyzed this origin by 2D gel

Poloumienko et al. (2001): Chr3:222368-226808

TourriÃ¨re et al. (2005): Chr3:222374-226803

Studies that detected this origin by chromatin immunoprecipitation (ChIP)

Wyrick et al. (2001): Chr3:223463-224292

Xu et al. (2006): Chr3:223725-225575

Shor et al. (2009): Chr3:224300-225300 (orc2-1/wt peak ratio: 0.66)

Szilard et al. (2010): Chr3:224975-224985

MÃ¼ller et al. (2010): Chr3:224300-225125 (orc1-bah-delta/wt peak ratio: 0.76)

Studies that measured the replication time of this origin

Raghuraman et al. (2001): Chr3:227557 (Trep: 14.8 min.) (Confidence: 9)

Yabuki et al. (2002): Chr3:222863 (Trep: 24.2 min.)

Alvino et al. (2007): Chr3:221330 (Peak first observed at 10.0 min.)

Studies that measured the activity of this origin in hydroxyurea (HU)

Feng et al. (2006): Chr3:224666 (Activity detected in: wild-type, rad53)

CrabbÃ© et al. (2010): Chr3:224807-225053 (Activity detected in: wild-type, rad9, rev3 rad30, eco1, ctf4, ddc1, rad24, pol2-12, elg1, tof1, mrc1-AQ, mrc1-AQ rad9, ctf8, ctf18, mrc1, dcc1, ctf18 rad9, mec1-100, rad53, mec1)