OriDB Curated Paper

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The conserved bromo-adjacent homology domain of yeast Orc1 functions in the selection of DNA replication origins within chromatin.

Philipp Müller, Sookhee Park, Erika Shor, Dana J Huebert, Christopher L Warren, Aseem Z Ansari, Michael Weinreich, Matthew L Eaton, David M MacAlpine, Catherine A Fox

Genes Dev. (2010), 24(13):1418-33Data to DownloadPubMed | PubMed Central | Genes Dev.

The origin recognition complex (ORC) binds to the specific positions on chromosomes that serve as DNA replication origins. Although ORC is conserved from yeast to humans, the DNA sequence elements that specify ORC binding are not. In particular, metazoan ORC shows no obvious DNA sequence specificity, whereas yeast ORC binds to a specific DNA sequence within all yeast origins. Thus, whereas chromatin must play an important role in metazoan ORC's ability to recognize origins, it is unclear whether chromatin plays a role in yeast ORC's recognition of origins. This study focused on the role of the conserved N-terminal bromo-adjacent homology domain of yeast Orc1 (Orc1BAH). Recent studies indicate that BAH domains are chromatin-binding modules. We show that the Orc1BAH domain was necessary for ORC's stable association with yeast chromosomes, and was physiologically relevant to DNA replication in vivo. This replication role was separable from the Orc1BAH domain's previously defined role in transcriptional silencing. Genome-wide analyses of ORC binding in ORC1 and orc1bahDelta cells revealed that the Orc1BAH domain contributed to ORC's association with most yeast origins, including a class of origins highly dependent on the Orc1BAH domain for ORC association (orc1bahDelta-sensitive origins). Orc1bahDelta-sensitive origins required the Orc1BAH domain for normal activity on chromosomes and plasmids, and were associated with a distinct local nucleosome structure. These data provide molecular insights into how the Orc1BAH domain contributes to ORC's selection of replication origins, as well as new tools for examining conserved mechanisms governing ORC's selection of origins within eukaryotic chromosomes.

OriDB annotation of this paper:

ARS assay

None curated.

2D gel

None curated.

ChIP of replication origin proteins

ARS102.5, ARS at V-510, ARS1624, ARS at III-308, ARS1323, ARS1211, ARS at III-308, ARS1223, ARS at XII-599, ARS1218, ARS at VII-33, ARS at VIII-553, ARS446, ARS at XIV-9, ARS at V-563, ARS at IX-407, ARS430, ARS1311.7, ARS at XV-354, ARS507, ARS1413, ARS213, ARS432, ARS504.2, ARS1628, ARS1216.5, ARS at XII-461, ARS1315, ARS at IX-183, ARS at XII-468, ARS at III-146, ARS1529.5, ARS1114.5, ARS1235, ARS at XII-1056, ARS603.1, ARS419, ARS412, ARS at XIV-577, ARS522, ARS306, ARS733, ARS432.5, ARS728, ARS215, ARS225, ARS822, ARS302, ARS303, ARS320, ARS302, ARS302, ARS303, ARS320, ARS302, ARS at XII-1020, ARS1234, ARS1327, ARS at XIII-763, ARS1309, ARS1620.5, ARS at I-206, ARS816, ARS at II-695, ARS at II-259, ARS222, ARS1609, ARS603.5, ARS at III-146, ARS at VIII-11, ARS805.5, ARS1022, ARS511, ARS1417, ARS106, ARS1631, ARS820, ARS at III-5, ARS at XVI-318, ARS1415, ARS at XVI-262, ARS1424, ARS1507, ARS at XV-79, ARS1217, ARS414, ARS at XIV-773, ARS309, ARS605, ARS1510.5, ARS214, ARS1429, ARS909, ARS at XII-140, ARS420, ARS1525, ARS at XVI-369, ARS517, ARS307, ARS518, ARS at VIII-367, ARS at XV-11, ARS704, ARS415, ARS417, ARS1109, ARS316, ARS610, ARS at VI-269, ARS at XIV-773, ARS707, ARS731.5, ARS at VII-8, ARS at VII-0, ARS at VII-8, ARS at VII-0, ARS805, ARS1232, ARS207.5, ARS1514, ARS109, ARS437, ARS at IV-1356, ARS1312, ARS at XIII-373, ARS at XVI-553, ARS1213, ARS417.5, ARS1004, ARS1427, ARS at XVI-454, ARS1622.7, ARS1510, ARS at VIII-116, ARS1106.7, ARS600.3, ARS600.4, ARS601, ARS602, ARS1414, ARS1212, ARS at II-266, ARS916, ARS1210, ARS1227, ARS1006, ARS1604, ARS314, ARS at III-200, ARS1511, ARS1310, ARS434, ARS at IV-1275, ARS920, ARS818, ARS1520, ARS1621, ARS1216, ARS406, ARS1220, ARS at XII-31, ARS513.7, ARS at I-230, ARS at I-17, ARS at V-317, ARS1515.5, ARS911, ARS1116, ARS1524, ARS at II-229, ARS208, ARS at II-246, ARS at XI-463, ARS1307.5, ARS516, ARS1219, ARS at XII-618, ARS1330, ARS209, ARS919, ARS1407, ARS912, ARS at XII-1078, ARS1412, ARS1614, ARS905, ARS447, ARS at IV-1499, ARS1222, ARS729, ARS1322, ARS at XVI-560, ARS1622, ARS1622.5, ARS431, ARS731, ARS1113, ARS at IX-198, ARS1405, ARS at XIV-36, ARS423, ARS104, ARS907, ARS435, ARS at VII-275, ARS702, ARS at XI-662, ARS718, ARS107, ARS1513.5, ARS220, ARS110, ARS at XVI-1, ARS913, ARS1605, ARS at XII-1071, ARS609, ARS1304, ARS1426, ARS433, ARS416, ARS421, ARS503, ARS1419, ARS428, ARS1009, ARS at XV-763, ARS1523, ARS1120, ARS at IX-439, ARS809, ARS1209, ARS512, ARS300, ARS404, ARS1104.5, ARS105, ARS1011, ARS1325, ARS1528, ARS734, ARS219, ARS1112, ARS at III-163, ARS310, ARS1626.5, ARS1303, ARS1107, ARS at XV-900, ARS at XI-633, ARS425, ARS305, ARS at XII-7, ARS1012, ARS1013, ARS815, ARS607, ARS510, ARS1114, ARS1320, ARS508, ARS1623, ARS922, ARS1324, ARS440, ARS714, ARS824, ARS1406, ARS at IX-8, ARS131n, ARS802, ARS523, ARS1002, ARS413, ARS1519, ARS at VIII-261, ARS1332, ARS1421, ARS1509, ARS1215, ARS1005, ARS1326, ARS1517, ARS at XV-605, ARS1103, ARS1422, ARS at XIV-556, ARS301, ARS302, ARS1021, ARS at XVI-942, ARS at XI-662, ARS600.1, ARS131a, ARS720, ARS1508, ARS1227.5, ARS at XVI-873, ARS1630, ARS1513, ARS at XVI-947, ARS1118, ARS200, ARS at V-563, ARS735.5, ARS at IV-1524, ARS at XIII-923, ARS1229, ARS at XII-1, ARS1018, ARS1619, ARS102, ARS111, ARS at VIII-562, ARS400, ARS228, ARS319, ARS318, ARS807, ARS at VIII-135, ARS202, ARS at XIV-1, ARS1618, ARS700.5, ARS317, ARS at XII-1064, ARS802, ARS at VII-1090, ARS at II-812, ARS1123, ARS1116, ARS at XIII-6, ARS908, ARS823.5, ARS1607, ARS1411, ARS at XIII-1, ARS at XV-1, ARS at XI-1, ARS at IX-1, ARS at XIV-9, ARS at IV-1531, ARS1015, ARS at IX-190, ARS1319, ARS at XVI-163, ARS at XIV-783, ARS at IX-238, ARS913.5, ARS914, ARS at IX-249, ARS at IX-238, ARS913.5, ARS914, ARS at IX-249, ARS1329, ARS409, ARS at IV-220, ARS at XII-12, ARS at III-224, ARS315, ARS1007, ARS405, ARS719, ARS at XV-1081, ARS1019, ARS1001, ARS201, ARS1512, ARS1516, ARS1025, ARS727, ARS at XV-1091, ARS1601, ARS207, ARS603, ARS1106, ARS1307, ARS1206, ARS1428.5, ARS at VIII-1, ARS206, ARS216, ARS at III-193, ARS313, ARS1008, ARS at X-212, ARS224, ARS1625, ARS429, ARS418, ARS442, ARS520, ARS1420, ARS813, ARS at V-576, ARS441, ARS422, ARS1627, ARS1211.5, ARS at V-1, ARS203, ARS514, ARS722, ARS at VII-576, ARS1226, ARS410, ARS1014, ARS1526, ARS513.5, ARS1010, ARS1626, ARS1521, ARS at XVI-440, ARS1308, ARS at VIII-88, ARS804, ARS716, ARS604

Replication timing

None curated.

Replication in hydroxyurea

None curated.

Predicted origins

None curated.

Confirmed sequence element

None curated.

Predicted sequence element

None curated.

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