Dubious ARS at III-5

No systematic name assigned

Other names: proARS300.5

Status: Dubious ARS: Identified by 4 genome-wide studies.

Genomic Location: Chr3:3814-5892
Probably within intergenic space between chromosome start and YCL073C.
• View at UCSC genome browser
• View on SGD Chromosome Features Map
• View at SGD gbrowser site
• View at Ensembl browser

DNA Sequence:

Origin Sequence Elements: No elements have been reported for this origin.

Helical Stability: Minimum -156.8 ΔG° (kcal/mol) at location 5241.

Time of Origin Replication (Trep): This site was not identified as an origin by any curated timing study (Raghuraman et al. (2001); Yabuki et al. (2002); Alvino et al. (2007)), suggesting that this site is not chromosomally active for replication initiation.

Origin activity in HU: Origin activity not detected in HU.

ARS at III-5 has unique ID: 64

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ARS at III-5 has unique ID: 64

Studies that cloned this origin

None curated.


Studies that analyzed this origin by 2D gel

None curated.


Studies that detected this origin by chromatin immunoprecipitation (ChIP)

Wyrick et al. (2001): Chr3:4314-5392

Xu et al. (2006): Chr3:25-17053

Shor et al. (2009): Chr3:300-8500 (orc2-1/wt peak ratio: 0.87)

Müller et al. (2010): Chr3:3725-5225 (orc1-bah-delta/wt peak ratio: 0.39)


Studies that measured the replication time of this origin

The replication time of origin has not be reported by any curated study.


Studies that measured the activity of this origin in hydroxyurea (HU)

This origin has not be reported as active in HU by any curated study.


Studies that predicted the location of this origin

This origin has not been predicted by any curated study.



ARS at III-5 has unique ID: 64

Studies that confirmed an essential ACS element

None curated.


Studies that predicted an essential ACS element

None curated.

ACS LOGO:   ACS_logo  


ARS at III-5 has unique ID: 64

Alignments from the UCSC genome browser

No phylogenetic sequence conservation data.

ARS at III-5 has unique ID: 64

These notes are manually curated. To submit notes for this replication origin site please contact us.

Friday, 18th August 2006 - Conrad Nieduszynski:

Carol Newlon's lab have checked this region for ARS activity and found none. [Thanks to Carol Newlon for this information.]

ARS at III-5 has unique ID: 64

Genome-wide studies that identified this origin

Wyrick et al. (2001): PubMed | Science

Xu et al. (2006): PubMed | PubMed Central | BMC Genomics

Shor et al. (2009): PubMed | PubMed Central | PLoS Genet.

Müller et al. (2010): PubMed | PubMed Central | Genes Dev.


Studies that cloned this origin

None identified.


Studies that analyzed this origin by 2D gel

None identified.


Studies that confirmed an essential ACS element

None identified.


Studies that predicted an essential ACS element

None identified.


ARS at III-5 has unique ID: 64