Confirmed ARS at VI-105

ARS603.1

Status: Confirmed ARS: Confirmed by ARS assay and identified by 5 genome-wide studies.

Genomic Location: Chr6:104456-104695
Within tandem intergenic space between YFL017C and YFL016C.
• View at UCSC genome browser
• View on SGD Chromosome Features Map
• View at SGD gbrowser site
• View at Ensembl browser

DNA Sequence:

Origin Sequence Elements: ACS predicted.

Helical Stability: Minimum -148.2 ΔG° (kcal/mol) at location 104456.

Time of Origin Replication (Trep): Alvino et al. (2007) — Peak first observed at 12.5 min.

Origin activity in HU: Origin activity detected in HU — more details.

ARS at VI-105 has unique ID: 682

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ARS at VI-105 has unique ID: 682

Studies that cloned this origin

Xu et al. (2006): Chr6:104456-104695


Studies that analyzed this origin by 2D gel

None curated.


Studies that detected this origin by chromatin immunoprecipitation (ChIP)

Xu et al. (2006): Chr6:104150-105055

Shor et al. (2009): Chr6:104800-105800 (orc2-1/wt peak ratio: 0.94)

Müller et al. (2010): Chr6:104150-104450 (orc1-bah-delta/wt peak ratio: 0.27)


Studies that measured the replication time of this origin

Alvino et al. (2007): Chr6:100000 (Peak first observed at 12.5 min.)


Studies that measured the activity of this origin in hydroxyurea (HU)

Crabbé et al. (2010): Chr6:104456-104695 (Activity detected in: wild-type, rad9, rev3 rad30, eco1, ctf4, ddc1, rad24, pol2-12, elg1, tof1, mrc1-AQ, mrc1-AQ rad9, ctf8, ctf18, mrc1, dcc1, ctf18 rad9, mec1-100, mec1)


Studies that predicted the location of this origin

This origin has not been predicted by any curated study.



ARS at VI-105 has unique ID: 682

Studies that confirmed an essential ACS element

None curated.


Studies that predicted an essential ACS element

  Xu et al. (2006):      TTTATTTATTTTTTTTTTTTTTGCGTAATACAAG  
ACS LOGO:   ACS_logo  

ARS at VI-105 has unique ID: 682

Alignments from the UCSC genome browser

No phylogenetic sequence conservation data.

ARS at VI-105 has unique ID: 682

These notes are manually curated. To submit notes for this replication origin site please contact us.

There are no notes entered for this replication origin site.

ARS at VI-105 has unique ID: 682

Genome-wide studies that identified this origin

Xu et al. (2006): PubMed | PubMed Central | BMC Genomics

Alvino et al. (2007): PubMed | PubMed Central | Mol. Cell. Biol.

Shor et al. (2009): PubMed | PubMed Central | PLoS Genet.

Müller et al. (2010): PubMed | PubMed Central | Genes Dev.

Crabbé et al. (2010): PubMed | Nat. Struct. Mol. Biol.


Studies that cloned this origin

Xu et al. (2006): PubMed | PubMed Central | BMC Genomics


Studies that analyzed this origin by 2D gel

None identified.


Studies that confirmed an essential ACS element

None identified.


Studies that predicted an essential ACS element

Xu et al. (2006): PubMed | PubMed Central | BMC Genomics


ARS at VI-105 has unique ID: 682