ARS1414

Status: Confirmed ARS: Confirmed by ARS assay and 2D gel and identified by 10 genome-wide studies.

Genomic Location: Chr14:279875-280108
Within tandem intergenic space between YNL192W and YNL191W.
• View at UCSC genome browser
• View on SGD Chromosome Features Map
• View at SGD gbrowser site
• View at Ensembl browser

DNA Sequence: View sequence

Origin Sequence Elements: ACS predicted.

Helical Stability: Minimum -147.2 ΔG° (kcal/mol) at location 280075.

Time of Origin Replication (Trep): Raghuraman et al. (2001) — Trep: 24.7 min.
Yabuki et al. (2002) — Trep: 25.9 min.
Alvino et al. (2007) — Peak first observed at 12.5 min.

Origin activity in HU: Origin activity detected in HU — more details.

ARS at XIV-280 has unique ID: ARS1414

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ARS at XIV-280 has unique ID: ARS1414

Studies that cloned this origin

Nieduszynski et al. (2006): Chr14:279875-280108

Studies that analyzed this origin by 2D gel

Friedman et al. (1996): Chr14:277746-281725

Studies that detected this origin by chromatin immunoprecipitation (ChIP)

Wyrick et al. (2001): Chr14:279893-280428

Xu et al. (2006): Chr14:279285-281255

Shor et al. (2009): Chr14:279300-280700 (orc2-1/wt peak ratio: 0.79)

Szilard et al. (2010): Chr14:280015-280025

Müller et al. (2010): Chr14:279350-280700 (orc1-bah-delta/wt peak ratio: 0.51)

Studies that measured the replication time of this origin

Raghuraman et al. (2001): Chr14:279803 (Trep: 24.7 min.) (Confidence: 9)

Yabuki et al. (2002): Chr14:282469 (Trep: 25.9 min.)

Alvino et al. (2007): Chr14:281000 (Peak first observed at 12.5 min.)

Studies that measured the activity of this origin in hydroxyurea (HU)

Feng et al. (2006): Chr14:279666 (Activity detected in: rad53)

Crabbé et al. (2010): Chr14:279875-280108 (Activity detected in: ddc1, pol2-12, elg1, tof1, mrc1-AQ, mrc1-AQ rad9, ctf8, ctf18, mrc1, dcc1, ctf18 rad9, mec1-100, rad53, mec1)

Studies that predicted the location of this origin

This origin has not been predicted by any curated study.

ARS at XIV-280 has unique ID: ARS1414

Studies that confirmed an essential ACS element

None curated.

Studies that predicted an essential ACS element

Nieduszynski et al. (2006):

T

T

A

T

T

T

A

T

G

T

T

T

T

T

C

T

Xu et al. (2006):

T

C

A

A

T

T

A

T

T

T

A

T

G

T

T

T

T

T

C

T

C

T

C

A

G

A

A

G

C

C

A

T

T

T

ACS LOGO:

ARS at XIV-280 has unique ID: ARS1414

Alignments from the UCSC genome browser

ARS at XIV-280 has unique ID: ARS1414

These notes are manually curated. To submit notes for this replication origin site please contact us.

There are no notes entered for this replication origin site.

ARS at XIV-280 has unique ID: ARS1414

Genome-wide studies that identified this origin

Raghuraman et al. (2001): PubMed | Science

Wyrick et al. (2001): PubMed | Science

Yabuki et al. (2002): PubMed

Feng et al. (2006): PubMed | PubMed Central | Nat. Cell Biol.

Xu et al. (2006): PubMed | PubMed Central | BMC Genomics

Alvino et al. (2007): PubMed | PubMed Central | Mol. Cell. Biol.

Shor et al. (2009): PubMed | PubMed Central | PLoS Genet.

Szilard et al. (2010): PubMed | PubMed Central | Nat. Struct. Mol. Biol.

Müller et al. (2010): PubMed | PubMed Central | Genes Dev.

Crabbé et al. (2010): PubMed | Nat. Struct. Mol. Biol.

Studies that cloned this origin

Nieduszynski et al. (2006): PubMed | PubMed Central | Genes Dev.

Studies that analyzed this origin by 2D gel

Friedman et al. (1996): PubMed

Studies that confirmed an essential ACS element

None identified.

Studies that predicted an essential ACS element

Nieduszynski et al. (2006): PubMed | PubMed Central | Genes Dev.

Xu et al. (2006): PubMed | PubMed Central | BMC Genomics

ARS at XIV-280 has unique ID: ARS1414