OriDB Curated Paper

Multiple determinants controlling activation of yeast replication origins late in S phase.

K L Friedman, J D Diller, B M Ferguson, S V Nyland, B J Brewer, W L Fangman

Genes Dev. (1996), 10(13):1595-607PubMed

Analysis of a 131-kb segment of the left arm of yeast chromosome XIV beginning 157 kb from the telomere reveals four highly active origins of replication that initiate replication late in S phase. Previous work has shown that telomeres act as determinants for late origin activation. However, at least two of the chromosome XIV origins maintain their late activation time when located on large circular plasmids, indicating that late replication is independent of telomeres. Analysis of the replication time of plasmid derivatives containing varying amounts of chromosome XIV DNA show that a minimum of three chromosomal elements, distinct from each tested origin, contribute to late activation time. These late determinants are functionally equivalent, because duplication of one set of contributing sequences can compensate for the removal of another set. Furthermore, insertion of an origin that is normally early activated into this domain results in a shift to late activation, suggesting that the chromosome XIV origins are not unique in their ability to respond to the late determinants.

OriDB annotation of this paper:

ARS assay

None curated.

2D gel

ARS1411, ARS1412, ARS1413, ARS1414

ChIP of replication origin proteins

None curated.

Replication timing

None curated.

Replication in hydroxyurea

None curated.

Predicted origins

None curated.

Confirmed sequence element

ARS1413

Predicted sequence element

None curated.