No systematic name assigned

Status: Dubious ARS: Identified by 5 genome-wide studies.

Genomic Location: Chr8:451493-453397
Probably within tandem intergenic space between YHR174W and YHR175W.
• View at UCSC genome browser
• View on SGD Chromosome Features Map
• View at SGD gbrowser site
• View at Ensembl browser

DNA Sequence: View sequence

Origin Sequence Elements: No elements have been reported for this origin.

Helical Stability: Minimum -165.2 ΔG° (kcal/mol) at location 451613.

Time of Origin Replication (Trep): Raghuraman et al. (2001) — Trep: 25.4 min.
Yabuki et al. (2002) — Trep: 20.9 min.
Alvino et al. (2007) — Peak first observed at 12.5 min.

Origin activity in HU: Origin activity not detected in HU.

ARS at VIII-452 has unique ID: 700

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ARS at VIII-452 has unique ID: 700

Studies that cloned this origin

None curated.

Studies that analyzed this origin by 2D gel

None curated.

Studies that detected this origin by chromatin immunoprecipitation (ChIP)

Xu et al. (2006): Chr8:451495-453395

Shor et al. (2009): Chr8:451400-452900 (orc2-1/wt peak ratio: 1.09)

Studies that measured the replication time of this origin

Raghuraman et al. (2001): Chr8:447627 (Trep: 25.4 min.) (Confidence: 9)

Yabuki et al. (2002): Chr8:447835 (Trep: 20.9 min.)

Alvino et al. (2007): Chr8:446780 (Peak first observed at 12.5 min.)

Studies that measured the activity of this origin in hydroxyurea (HU)

This origin has not be reported as active in HU by any curated study.

Studies that predicted the location of this origin

This origin has not been predicted by any curated study.

ARS at VIII-452 has unique ID: 700

Studies that confirmed an essential ACS element

None curated.

Studies that predicted an essential ACS element

None curated.

ACS LOGO:

ARS at VIII-452 has unique ID: 700

Alignments from the UCSC genome browser

ARS at VIII-452 has unique ID: 700

These notes are manually curated. To submit notes for this replication origin site please contact us.

Friday, 4th December 2009 - Conrad Nieduszynski:

Status manually changed to 'Dubious'. Shor et al. (2009) report that there is no detectable ARS activity in this region.

ARS at VIII-452 has unique ID: 700

Genome-wide studies that identified this origin

Raghuraman et al. (2001): PubMed | Science

Yabuki et al. (2002): PubMed

Xu et al. (2006): PubMed | PubMed Central | BMC Genomics

Alvino et al. (2007): PubMed | PubMed Central | Mol. Cell. Biol.

Shor et al. (2009): PubMed | PubMed Central | PLoS Genet.

Studies that cloned this origin

None identified.

Studies that analyzed this origin by 2D gel

None identified.

Studies that confirmed an essential ACS element

None identified.

Studies that predicted an essential ACS element

None identified.

ARS at VIII-452 has unique ID: 700