Likely ARS at IV-444

No systematic name assigned

Status: Likely ARS: Identified by 4 genome-wide studies.

Genomic Location: Chr4:443318-444447
Probably within tandem intergenic space between YDL004W and YDL003W.
View at UCSC genome browser
View on SGD Chromosome Features Map
View at SGD gbrowser site
View at Ensembl browser

DNA Sequence:

Origin Sequence Elements: ACS predicted.

Helical Stability: Minimum -152.9 ΔG° (kcal/mol) at location 444338.

Time of Origin Replication (Trep): Raghuraman et al. (2001) — Trep: 20.2 min.
Alvino et al. (2007) — Peak first observed at 12.5 min.

Origin activity in HU: Origin activity detected in HU — more details.

ARS at IV-444 has unique ID: 670

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ARS at IV-444 has unique ID: 670

Studies that cloned this origin

None curated.


Studies that analyzed this origin by 2D gel

None curated.


Studies that detected this origin by chromatin immunoprecipitation (ChIP)

Xu et al. (2006): Chr4:443320-444445


Studies that measured the replication time of this origin

Raghuraman et al. (2001): Chr4:442864 (Trep: 20.2 min.) (Confidence: 1)

Alvino et al. (2007): Chr4:437000 (Peak first observed at 12.5 min.)


Studies that measured the activity of this origin in hydroxyurea (HU)

Crabbé et al. (2010): Chr4:443318-444447 (Activity detected in: wild-type, rad9, rev3 rad30, eco1, ctf4, ddc1, rad24, pol2-12, elg1, tof1, mrc1-AQ, mrc1-AQ rad9, ctf8, ctf18, mrc1, dcc1, ctf18 rad9, mec1-100, rad53, mec1)


Studies that predicted the location of this origin

This origin has not been predicted by any curated study.



ARS at IV-444 has unique ID: 670

Studies that confirmed an essential ACS element

None curated.


Studies that predicted an essential ACS element

  Xu et al. (2006):      ACATGTTAATGTTTTCTCTTTTGTTTTCTTGTTT  
  Xu et al. (2006):      AAGTTTCCATTTTGGTTGATGTCTTAAGTATACA  
ACS LOGO:   ACS_logo  

ARS at IV-444 has unique ID: 670

Alignments from the UCSC genome browser

No phylogenetic sequence conservation data.

ARS at IV-444 has unique ID: 670

These notes are manually curated. To submit notes for this replication origin site please contact us.

There are no notes entered for this replication origin site.

ARS at IV-444 has unique ID: 670

Genome-wide studies that identified this origin

Raghuraman et al. (2001): PubMed | Science

Xu et al. (2006): PubMed | PubMed Central | BMC Genomics

Alvino et al. (2007): PubMed | PubMed Central | Mol. Cell. Biol.

Crabbé et al. (2010): PubMed | Nat. Struct. Mol. Biol.


Studies that cloned this origin

None identified.


Studies that analyzed this origin by 2D gel

None identified.


Studies that confirmed an essential ACS element

None identified.


Studies that predicted an essential ACS element

Xu et al. (2006): PubMed | PubMed Central | BMC Genomics


ARS at IV-444 has unique ID: 670