OriDB - ARS432

Status: Confirmed ARS: Confirmed by ARS assay and identified by 9 genome-wide studies.

Genomic Location: Chr4:1159250-1159499
Within tandem intergenic space between YDR342C and YDR343C.
• View at UCSC genome browser
• View on SGD Chromosome Features Map
• View at SGD gbrowser site
• View at Ensembl browser

DNA Sequence:

Origin Sequence Elements: ACS confirmed.

Helical Stability: Minimum -148.6 ΔG° (kcal/mol) at location 1159330.

Time of Origin Replication (Trep): Raghuraman et al. (2001) — Trep: 12.6 min.
Yabuki et al. (2002) — Trep: 17.0 min.
Alvino et al. (2007) — Peak first observed at 10.0 min.

Origin activity in HU: Origin activity detected in HU — more details.

ARS at IV-1159 has unique ID: ARS432

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ARS at IV-1159 has unique ID: ARS432

Studies that cloned this origin

Nieduszynski et al. (2006): Chr4:1159250-1159499

Studies that analyzed this origin by 2D gel

None curated.

Studies that detected this origin by chromatin immunoprecipitation (ChIP)

Wyrick et al. (2001): Chr4:1158415-1159643

Xu et al. (2006): Chr4:1158100-1159700

Shor et al. (2009): Chr4:1159000-1159800 (orc2-1/wt peak ratio: 0.46)

Szilard et al. (2010): Chr4:1159385-1159395

Müller et al. (2010): Chr4:1159025-1159775 (orc1-bah-delta/wt peak ratio: 0.24)

Studies that measured the replication time of this origin

Raghuraman et al. (2001): Chr4:1164550 (Trep: 12.6 min.) (Confidence: 9)

Yabuki et al. (2002): Chr4:1162291 (Trep: 17.0 min.)

Alvino et al. (2007): Chr4:1162400 (Peak first observed at 10.0 min.)

Studies that measured the activity of this origin in hydroxyurea (HU)

Crabbé et al. (2010): Chr4:1159250-1159499 (Activity detected in: wild-type, rad9, rev3 rad30, eco1, ctf4, ddc1, rad24, pol2-12, elg1, tof1, mrc1-AQ, mrc1-AQ rad9, ctf8, ctf18, mrc1, dcc1, ctf18 rad9, mec1-100, rad53, mec1)

Studies that predicted the location of this origin

This origin has not been predicted by any curated study.

ARS at IV-1159 has unique ID: ARS432

Studies that confirmed an essential ACS element

Nieduszynski et al. (2006):

A

T

C

T

C

T

Studies that predicted an essential ACS element

None curated.

ACS LOGO:

ARS at IV-1159 has unique ID: ARS432

Alignments from the UCSC genome browser

Confirmed ACS

S. cerevisiae

-

T

A

T

A

T

C

T

C

T

A

G

C

T

G

A

G

A

G

T

A

S. kudriavzevii

-

T

A

T

C

T

C

T

C

A

G

C

T

G

A

G

A

G

T

C

A

S. mikatae

-

T

A

T

A

T

G

T

C

T

C

T

A

G

T

G

A

G

A

T

A

T

A

S. paradoxus

-

T

A

T

A

T

C

T

C

T

C

T

A

G

C

T

G

A

G

A

G

A

G

T

A

S. bayanus

-

T

A

C

T

A

T

A

T

C

T

A

T

A

C

G

C

A

T

G

C

G

A

G

A

G

T

C

A

*

:

*

:

*

:

*

:

*

:

*

:

*

ARS at IV-1159 has unique ID: ARS432

These notes are manually curated. To submit notes for this replication origin site please contact us.

There are no notes entered for this replication origin site.

ARS at IV-1159 has unique ID: ARS432

Genome-wide studies that identified this origin

Raghuraman et al. (2001): PubMed | Science

Wyrick et al. (2001): PubMed | Science

Yabuki et al. (2002): PubMed

Xu et al. (2006): PubMed | PubMed Central | BMC Genomics

Alvino et al. (2007): PubMed | PubMed Central | Mol. Cell. Biol.

Shor et al. (2009): PubMed | PubMed Central | PLoS Genet.

Szilard et al. (2010): PubMed | PubMed Central | Nat. Struct. Mol. Biol.

Müller et al. (2010): PubMed | PubMed Central | Genes Dev.

Crabbé et al. (2010): PubMed | Nat. Struct. Mol. Biol.

Studies that cloned this origin

Nieduszynski et al. (2006): PubMed | PubMed Central | Genes Dev.

Studies that analyzed this origin by 2D gel

None identified.

Studies that confirmed an essential ACS element

Nieduszynski et al. (2006): PubMed | PubMed Central | Genes Dev.

Studies that predicted an essential ACS element

None identified.

ARS at IV-1159 has unique ID: ARS432

Confirmed ARS at IV-1159

ARS432

Other names: proARS432

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Studies that cloned this origin

Studies that analyzed this origin by 2D gel

Studies that detected this origin by chromatin immunoprecipitation (ChIP)

Studies that measured the replication time of this origin

Studies that measured the activity of this origin in hydroxyurea (HU)

Studies that predicted the location of this origin

Studies that confirmed an essential ACS element

Studies that predicted an essential ACS element

Alignments from the UCSC genome browser

Genome-wide studies that identified this origin

Studies that cloned this origin

Studies that analyzed this origin by 2D gel

Studies that confirmed an essential ACS element

Studies that predicted an essential ACS element