ARS731.5

Status: Confirmed ARS: Confirmed by ARS assay and identified by 9 genome-wide studies.

Genomic Location: Chr7:888350-888599
Within divergent intergenic space between YGR194C and YGR195W.
• View at UCSC genome browser
• View on SGD Chromosome Features Map
• View at SGD gbrowser site
• View at Ensembl browser

DNA Sequence: View sequence

Origin Sequence Elements: ACS confirmed.

Helical Stability: Minimum -149.6 ΔG° (kcal/mol) at location 888580.

Time of Origin Replication (Trep): Raghuraman et al. (2001) — Trep: 21.8 min.
Yabuki et al. (2002) — Trep: 16.8 min.
Alvino et al. (2007) — Peak first observed at 10.0 min.

Origin activity in HU: Origin activity detected in HU — more details.

ARS at VII-888 has unique ID: 253

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ARS at VII-888 has unique ID: 253

Studies that cloned this origin

Nieduszynski et al. (2006): Chr7:888350-888599

Studies that analyzed this origin by 2D gel

None curated.

Studies that detected this origin by chromatin immunoprecipitation (ChIP)

Xu et al. (2006): Chr7:887165-889585

Shor et al. (2009): Chr7:887800-889000 (orc2-1/wt peak ratio: 0.36)

Szilard et al. (2010): Chr7:888515-888525

Müller et al. (2010): Chr7:887450-889025 (orc1-bah-delta/wt peak ratio: 0.46)

Studies that measured the replication time of this origin

Raghuraman et al. (2001): Chr7:891133 (Trep: 21.8 min.) (Confidence: 9)

Yabuki et al. (2002): Chr7:890147 (Trep: 16.8 min.)

Alvino et al. (2007): Chr7:889670 (Peak first observed at 10.0 min.)

Studies that measured the activity of this origin in hydroxyurea (HU)

Feng et al. (2006): Chr7:889250 (Activity detected in: wild-type, rad53)

Crabbé et al. (2010): Chr7:888350-888599 (Activity detected in: wild-type, rad9, rev3 rad30, eco1, ctf4, ddc1, rad24, pol2-12, elg1, tof1, mrc1-AQ, mrc1-AQ rad9, ctf8, ctf18, mrc1, dcc1, ctf18 rad9, mec1-100, rad53, mec1)

Studies that predicted the location of this origin

This origin has not been predicted by any curated study.

ARS at VII-888 has unique ID: 253

Studies that confirmed an essential ACS element

Studies that predicted an essential ACS element

Xu et al. (2006):

C

T

T

A

A

T

T

T

T

T

C

T

T

T

T

T

T

G

T

T

T

A

T

G

T

T

T

T

A

T

T

T

C

C

Xu et al. (2006):

T

T

T

A

A

T

A

T

T

T

T

G

T

T

C

T

T

T

A

G

T

T

T

T

A

A

A

A

A

T

T

C

A

G

Eaton et al. (2010):

T

A

T

T

T

T

A

A

T

A

T

T

T

T

G

T

T

C

T

T

T

A

G

T

T

T

T

A

A

A

A

A

T

ACS LOGO:

ARS at VII-888 has unique ID: 253

Alignments from the UCSC genome browser

ARS at VII-888 has unique ID: 253

These notes are manually curated. To submit notes for this replication origin site please contact us.

There are no notes entered for this replication origin site.

ARS at VII-888 has unique ID: 253

Genome-wide studies that identified this origin

Raghuraman et al. (2001): PubMed | Science

Yabuki et al. (2002): PubMed

Feng et al. (2006): PubMed | PubMed Central | Nat. Cell Biol.

Xu et al. (2006): PubMed | PubMed Central | BMC Genomics

Alvino et al. (2007): PubMed | PubMed Central | Mol. Cell. Biol.

Shor et al. (2009): PubMed | PubMed Central | PLoS Genet.

Szilard et al. (2010): PubMed | PubMed Central | Nat. Struct. Mol. Biol.

Müller et al. (2010): PubMed | PubMed Central | Genes Dev.

Crabbé et al. (2010): PubMed | Nat. Struct. Mol. Biol.

Studies that cloned this origin

Nieduszynski et al. (2006): PubMed | PubMed Central | Genes Dev.

Studies that analyzed this origin by 2D gel

None identified.

Studies that confirmed an essential ACS element

Nieduszynski et al. (2006): PubMed | PubMed Central | Genes Dev.

Studies that predicted an essential ACS element

Xu et al. (2006): PubMed | PubMed Central | BMC Genomics

Eaton et al. (2010): PubMed | PubMed Central | Genes Dev.

ARS at VII-888 has unique ID: 253