ARS309

Status: Confirmed ARS: Confirmed by ARS assay and identified by 10 genome-wide studies.

Genomic Location: Chr3:131978-132322
Within tandem intergenic space between YCR009C and YCR010C.
• View at UCSC genome browser
• View on SGD Chromosome Features Map
• View at SGD gbrowser site
• View at Ensembl browser

DNA Sequence: View sequence

Origin Sequence Elements: ACS confirmed.

Helical Stability: Minimum -153.2 ΔG° (kcal/mol) at location 132298.

Time of Origin Replication (Trep): Raghuraman et al. (2001) — Trep: 22.5 min.
Yabuki et al. (2002) — Trep: 22.4 min.
Alvino et al. (2007) — Peak first observed at 10.0 min.

Origin activity in HU: Origin activity detected in HU — more details.

ARS at III-132 has unique ID: ARS309

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ARS at III-132 has unique ID: ARS309

Studies that cloned this origin

Palzkill et al. (1986): Chr3:131978-132322

Studies that analyzed this origin by 2D gel

None curated.

Studies that detected this origin by chromatin immunoprecipitation (ChIP)

Wyrick et al. (2001): Chr3:132289-132885

Xu et al. (2006): Chr3:131020-133615

Shor et al. (2009): Chr3:131700-133500 (orc2-1/wt peak ratio: 0.44)

Szilard et al. (2010): Chr3:132165-132175

Müller et al. (2010): Chr3:131600-132800 (orc1-bah-delta/wt peak ratio: 0.40)

Studies that measured the replication time of this origin

Raghuraman et al. (2001): Chr3:128423 (Trep: 22.5 min.) (Confidence: 2)

Yabuki et al. (2002): Chr3:133363 (Trep: 22.4 min.)

Alvino et al. (2007): Chr3:133710 (Peak first observed at 10.0 min.)

Studies that measured the activity of this origin in hydroxyurea (HU)

Feng et al. (2006): Chr3:131500 (Activity detected in: wild-type, rad53)

Crabbé et al. (2010): Chr3:131978-132322 (Activity detected in: wild-type, rad9, rev3 rad30, eco1, ctf4, ddc1, rad24, pol2-12, elg1, tof1, mrc1-AQ, mrc1-AQ rad9, ctf8, ctf18, mrc1, dcc1, ctf18 rad9, mec1-100, rad53, mec1)

Studies that predicted the location of this origin

This origin has not been predicted by any curated study.

ARS at III-132 has unique ID: ARS309

Studies that confirmed an essential ACS element

Studies that predicted an essential ACS element

Xu et al. (2006):

T

T

T

T

A

T

T

T

T

T

C

A

A

T

T

T

T

T

T

A

A

T

A

T

G

A

T

A

A

T

T

A

T

T

Xu et al. (2006):

G

T

T

T

A

T

A

T

C

T

T

G

T

T

T

T

G

A

A

G

T

T

T

T

A

G

C

T

T

T

G

A

A

A

Eaton et al. (2010):

A

T

T

G

T

T

T

A

T

A

T

C

T

T

G

T

T

T

T

G

A

A

G

T

T

T

T

A

G

C

T

T

T

ACS LOGO:

ARS at III-132 has unique ID: ARS309

Alignments from the UCSC genome browser

ARS at III-132 has unique ID: ARS309

These notes are manually curated. To submit notes for this replication origin site please contact us.

There are no notes entered for this replication origin site.

ARS at III-132 has unique ID: ARS309

Genome-wide studies that identified this origin

Raghuraman et al. (2001): PubMed | Science

Wyrick et al. (2001): PubMed | Science

Yabuki et al. (2002): PubMed

Feng et al. (2006): PubMed | PubMed Central | Nat. Cell Biol.

Xu et al. (2006): PubMed | PubMed Central | BMC Genomics

Alvino et al. (2007): PubMed | PubMed Central | Mol. Cell. Biol.

Shor et al. (2009): PubMed | PubMed Central | PLoS Genet.

Szilard et al. (2010): PubMed | PubMed Central | Nat. Struct. Mol. Biol.

Müller et al. (2010): PubMed | PubMed Central | Genes Dev.

Crabbé et al. (2010): PubMed | Nat. Struct. Mol. Biol.

Studies that cloned this origin

Palzkill et al. (1986): PubMed | PubMed Central

Studies that analyzed this origin by 2D gel

None identified.

Studies that confirmed an essential ACS element

Theis & Newlon (1997): PubMed | PubMed Central

Studies that predicted an essential ACS element

Xu et al. (2006): PubMed | PubMed Central | BMC Genomics

Eaton et al. (2010): PubMed | PubMed Central | Genes Dev.

ARS at III-132 has unique ID: ARS309