Confirmed ARS at X-100

ARS1006

Other names: proARS1006

Status: Confirmed ARS: Confirmed by ARS assay and identified by 8 genome-wide studies.

Genomic Location: Chr10:99359-99796
Within convergent intergenic space between YJL172W and YJL171C.
View at UCSC genome browser
View on SGD Chromosome Features Map
View at SGD gbrowser site
View at Ensembl browser

DNA Sequence:

Origin Sequence Elements: ACS predicted.

Helical Stability: Minimum -155.7 ΔG° (kcal/mol) at location 99749.

Time of Origin Replication (Trep): Yabuki et al. (2002) — Trep: 24.8 min.

Origin activity in HU: Origin activity detected in HU — more details.

ARS at X-100 has unique ID: ARS1006

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ARS at X-100 has unique ID: ARS1006

Studies that cloned this origin

Wyrick et al. (2001): Chr10:99359-99796


Studies that analyzed this origin by 2D gel

None curated.


Studies that detected this origin by chromatin immunoprecipitation (ChIP)

Wyrick et al. (2001): Chr10:99460-99697

Xu et al. (2006): Chr10:98352-100955

Shor et al. (2009): Chr10:98300-100700 (orc2-1/wt peak ratio: 0.47)

Szilard et al. (2010): Chr10:99415-99425

Müller et al. (2010): Chr10:99350-100625 (orc1-bah-delta/wt peak ratio: 0.51)


Studies that measured the replication time of this origin

Yabuki et al. (2002): Chr10:100933

Yabuki et al. (2002): Chr10:97433 (Trep: 24.8 min.)


Studies that measured the activity of this origin in hydroxyurea (HU)

Crabbé et al. (2010): Chr10:99359-99796 (Activity detected in: wild-type, rad9, rev3 rad30, eco1, ctf4, ddc1, rad24, pol2-12, elg1, tof1, mrc1-AQ, mrc1-AQ rad9, ctf8, ctf18, mrc1, dcc1, ctf18 rad9, mec1-100, mec1)


Studies that predicted the location of this origin

This origin has not been predicted by any curated study.



ARS at X-100 has unique ID: ARS1006

Studies that confirmed an essential ACS element

None curated.


Studies that predicted an essential ACS element

  Nieduszynski et al. (2006):       TTTTTTAATTTTTTTT                   
  Xu et al. (2006):      CATCATTTAAATTTTGATTTGACACTTTGATTTG  
  Xu et al. (2006):      ATGGTATTATATTTTGGCCATATTCACTGCCTTT  
  Xu et al. (2006):      TTTTATTTATGTTTTGTTTAACTGACGGTGTTTT  
  Xu et al. (2006):      TTTTTAATTTTTTTTTTTTAATTTTTTTTTTTTC  
  Eaton et al. (2010):      TTTATTTATGTTTTGTTTAACTGACGGTGTTTT   
ACS LOGO:   ACS_logo  

ARS at X-100 has unique ID: ARS1006

Alignments from the UCSC genome browser

Predicted ACS
S. cerevisiaeTGAATAGTCAAAGATTTTTTTTTTTTAATTTTTTTTTTTTAATTTTTTTTTTTTCA
S. kudriavzeviiTAAATAATCAAAAGAAC--------------TTTTTTTGTA--TTTTTTTTTTCCA
S. mikataeTGAATTGTAAAAG-----------------TTTTTTTTTCA------TTTTTTTCA
S. paradoxusTGAATAATTAAAG--------------AATTTTTTTTTTTAATTTTTTTTTTTTCA
S. bayanusCAAATAACCAAAAATCT----------TTTTTCTTTTTTTACCTAATATTTTT---
:***::***::::::::::::*:*****::*:::*****::

ARS at X-100 has unique ID: ARS1006

These notes are manually curated. To submit notes for this replication origin site please contact us.

There are no notes entered for this replication origin site.

ARS at X-100 has unique ID: ARS1006

Genome-wide studies that identified this origin

Wyrick et al. (2001): PubMed | Science

Yabuki et al. (2002): PubMed

Xu et al. (2006): PubMed | PubMed Central | BMC Genomics

Shor et al. (2009): PubMed | PubMed Central | PLoS Genet.

Szilard et al. (2010): PubMed | PubMed Central | Nat. Struct. Mol. Biol.

Müller et al. (2010): PubMed | PubMed Central | Genes Dev.

Crabbé et al. (2010): PubMed | Nat. Struct. Mol. Biol.


Studies that cloned this origin

Wyrick et al. (2001): PubMed | Science


Studies that analyzed this origin by 2D gel

None identified.


Studies that confirmed an essential ACS element

None identified.


Studies that predicted an essential ACS element

Nieduszynski et al. (2006): PubMed | PubMed Central | Genes Dev.

Xu et al. (2006): PubMed | PubMed Central | BMC Genomics

Eaton et al. (2010): PubMed | PubMed Central | Genes Dev.


ARS at X-100 has unique ID: ARS1006