Confirmed ARS at X-8

ARS1002

Other names: X-ARS, proARS1002

Status: Confirmed ARS: Confirmed by ARS assay and identified by 5 genome-wide studies.

Genomic Location: Chr10:7444-7943
Within tandem intergenic space between YJL225C and YJL223C.
View at UCSC genome browser
View on SGD Chromosome Features Map
View at SGD gbrowser site
View at Ensembl browser

DNA Sequence:

Origin Sequence Elements: ACS confirmed.

Helical Stability: Minimum -155.4 ΔG° (kcal/mol) at location 7501.

Time of Origin Replication (Trep): This site was not identified as an origin by any curated timing study (Raghuraman et al. (2001); Yabuki et al. (2002); Alvino et al. (2007)), suggesting that this site is not chromosomally active for replication initiation.

Origin activity in HU: Origin activity not detected in HU.

ARS at X-8 has unique ID: ARS1002

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ARS at X-8 has unique ID: ARS1002

Studies that cloned this origin

Wyrick et al. (2001): Chr10:7444-7943


Studies that analyzed this origin by 2D gel

None curated.


Studies that detected this origin by chromatin immunoprecipitation (ChIP)

Wyrick et al. (2001): Chr10:7453-8776

Xu et al. (2006): Chr10:6470-9729

Shor et al. (2009): Chr10:7100-9500 (orc2-1/wt peak ratio: 0.87)

Szilard et al. (2010): Chr10:7815-7825

Müller et al. (2010): Chr10:6725-9275 (orc1-bah-delta/wt peak ratio: 0.69)


Studies that measured the replication time of this origin

The replication time of origin has not be reported by any curated study.


Studies that measured the activity of this origin in hydroxyurea (HU)

This origin has not be reported as active in HU by any curated study.


Studies that predicted the location of this origin

This origin has not been predicted by any curated study.



ARS at X-8 has unique ID: ARS1002

Studies that confirmed an essential ACS element

  Xu et al. (2006):         TTTTTATGTTTA                     

Studies that predicted an essential ACS element

  Nieduszynski et al. (2006):       ATTTTTATGTTTAGGT                   
  Xu et al. (2006):      TATTTTTATGTTTAGGTGATTTTGGTGGTGATTT  
ACS LOGO:   ACS_logo  

ARS at X-8 has unique ID: ARS1002

Alignments from the UCSC genome browser

Predicted ACS
S. cerevisiaeTTATTGTTGAAGAATAGAATATTTTTATGTTTAGGTGATTTTGGTGGTGATTTTTA
S. kudriavzeviiTTCTTATTGATAATCAGTATATTTTTATGTTTGGGTAATTTTAGTGGTGATTGTT-
S. mikatae-------------------TATTTCTGTGTT-AGGTTATTTTGGTGGTGATTTTTC
S. paradoxus----TGGTGATATGTAGTATATTTTTATGTTTAGGTGATTTTAGTGGTGATTATTT
:::::::*****:*:****::*******************

ARS at X-8 has unique ID: ARS1002

These notes are manually curated. To submit notes for this replication origin site please contact us.

There are no notes entered for this replication origin site.

ARS at X-8 has unique ID: ARS1002

Genome-wide studies that identified this origin

Wyrick et al. (2001): PubMed | Science

Xu et al. (2006): PubMed | PubMed Central | BMC Genomics

Shor et al. (2009): PubMed | PubMed Central | PLoS Genet.

Szilard et al. (2010): PubMed | PubMed Central | Nat. Struct. Mol. Biol.

Müller et al. (2010): PubMed | PubMed Central | Genes Dev.


Studies that cloned this origin

Wyrick et al. (2001): PubMed | Science


Studies that analyzed this origin by 2D gel

None identified.


Studies that confirmed an essential ACS element

Xu et al. (2006): PubMed | PubMed Central | BMC Genomics


Studies that predicted an essential ACS element

Nieduszynski et al. (2006): PubMed | PubMed Central | Genes Dev.

Xu et al. (2006): PubMed | PubMed Central | BMC Genomics


ARS at X-8 has unique ID: ARS1002