Dubious ARS at III-85

No systematic name assigned

Status: Dubious ARS: Identified by 2 genome-wide studies.

Genomic Location: Chr3:81000-89000
Resolution insufficient to assign intergenic space.
• View at UCSC genome browser
• View on SGD Chromosome Features Map
• View at SGD gbrowser site
• View at Ensembl browser

DNA Sequence:

Origin Sequence Elements: No elements have been reported for this origin.

Helical Stability: Minimum -157.9 ΔG° (kcal/mol) at location 85320.

Time of Origin Replication (Trep): Raghuraman et al. (2001) — Trep: 10.8 min.

Origin activity in HU: Origin activity detected in HU — more details.

ARS at III-85 has unique ID: 72

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ARS at III-85 has unique ID: 72

Studies that cloned this origin

None curated.


Studies that analyzed this origin by 2D gel

None curated.


Studies that detected this origin by chromatin immunoprecipitation (ChIP)

None curated.


Studies that measured the replication time of this origin

Raghuraman et al. (2001): Chr3:77855 (Trep: 10.8 min.) (Confidence: 9)


Studies that measured the activity of this origin in hydroxyurea (HU)

Feng et al. (2006): Chr3:85000 (Activity detected in: wild-type, rad53)


Studies that predicted the location of this origin

This origin has not been predicted by any curated study.



ARS at III-85 has unique ID: 72

Studies that confirmed an essential ACS element

None curated.


Studies that predicted an essential ACS element

None curated.

ACS LOGO:   ACS_logo  


ARS at III-85 has unique ID: 72

Alignments from the UCSC genome browser

No phylogenetic sequence conservation data.

ARS at III-85 has unique ID: 72

These notes are manually curated. To submit notes for this replication origin site please contact us.

Friday, 18th August 2006 - Conrad Nieduszynski:

Status manually changed to 'Dubious'. Region contains repetitive DNA that may cross hybridizes giving rise to spurious microarray data. Furthermore, Carol Newlon's lab report that there is no detectable ARS activity in this region. [Thanks to Carol Newlon for this information.]

ARS at III-85 has unique ID: 72

Genome-wide studies that identified this origin

Raghuraman et al. (2001): PubMed | Science

Feng et al. (2006): PubMed | PubMed Central | Nat. Cell Biol.


Studies that cloned this origin

None identified.


Studies that analyzed this origin by 2D gel

None identified.


Studies that confirmed an essential ACS element

None identified.


Studies that predicted an essential ACS element

None identified.


ARS at III-85 has unique ID: 72