OriDB Curated Paper

H3 k36 methylation helps determine the timing of cdc45 association with replication origins.

Fiona Pryde, Devanshi Jain, Alastair Kerr, Rebecca Curley, Francesca Romana Mariotti, Maria Vogelauer

PLoS ONE (2009), 4(6):e5882PubMed | PubMed Central | PLoS ONE

Replication origins fire at different times during S-phase. Such timing is determined by the chromosomal context, which includes the activity of nearby genes, telomeric position effects and chromatin structure, such as the acetylation state of the surrounding chromatin. Activation of replication origins involves the conversion of a pre-replicative complex to a replicative complex. A pivotal step during this conversion is the binding of the replication factor Cdc45, which associates with replication origins at approximately their time of activation in a manner partially controlled by histone acetylation.

OriDB annotation of this paper:

ARS assay

None curated.

2D gel

None curated.

ChIP of replication origin proteins

None curated.

Replication timing

None curated.

Replication in hydroxyurea

None curated.

Predicted origins

None curated.

Confirmed sequence element

None curated.

Predicted sequence element

None curated.