Please contact OriDB if you think this paper has been mis-annotated.
Heather J McCune, Laura S Danielson, Gina M Alvino, David Collingwood, Jeffrey J Delrow, Walton L Fangman, Bonita J Brewer, M K Raghuraman
Genetics (2008), 180(4):1833-47Data to Download — PubMed | PubMed Central | Genetics
Temporal regulation of origin activation is widely thought to explain the pattern of early- and late-replicating domains in the Saccharomyces cerevisiae genome. Recently, single-molecule analysis of replication suggested that stochastic processes acting on origins with different probabilities of activation could generate the observed kinetics of replication without requiring an underlying temporal order. To distinguish between these possibilities, we examined a clb5Delta strain, where origin firing is largely limited to the first half of S phase, to ask whether all origins nonspecifically show decreased firing (as expected for disordered firing) or if only some origins ("late" origins) are affected. Approximately half the origins in the mutant genome show delayed replication while the remainder replicate largely on time. The delayed regions can encompass hundreds of kilobases and generally correspond to regions that replicate late in wild-type cells. Kinetic analysis of replication in wild-type cells reveals broad windows of origin firing for both early and late origins. Our results are consistent with a temporal model in which origins can show some heterogeneity in both time and probability of origin firing, but clustering of temporally like origins nevertheless yields a genome that is organized into blocks showing different replication times.
None curated.
None curated.
None curated.
None curated.
None curated.
None curated.
None curated.
None curated.
The data on this page come directly from PubMed and OriDB databases, please report any errors to OriDB.
This page is new! Please let us know of any problems you experience.