Confirmed ARS at XV-874

ARS1526

Other names: proARS1526

Status: Confirmed ARS: Confirmed by ARS assay and identified by 10 genome-wide studies.

Genomic Location: Chr15:874190-874434
Within convergent intergenic space between YOR296W and YOR297C.
View at UCSC genome browser
View on SGD Chromosome Features Map
View at SGD gbrowser site
View at Ensembl browser

DNA Sequence:

Origin Sequence Elements: ACS confirmed.

Helical Stability: Minimum -136.5 ΔG° (kcal/mol) at location 874420.

Time of Origin Replication (Trep): Raghuraman et al. (2001) — Trep: 19.4 min.
Yabuki et al. (2002) — Trep: 23.3 min.
Alvino et al. (2007) — Peak first observed at 12.5 min.

Origin activity in HU: Origin activity detected in HU — more details.

ARS at XV-874 has unique ID: 578

Loading - Please wait...

ARS at XV-874 has unique ID: 578

Studies that cloned this origin

Nieduszynski et al. (2006): Chr15:874190-874434


Studies that analyzed this origin by 2D gel

None curated.


Studies that detected this origin by chromatin immunoprecipitation (ChIP)

Wyrick et al. (2001): Chr15:874066-874738

Xu et al. (2006): Chr15:873455-875145

Shor et al. (2009): Chr15:874100-874800 (orc2-1/wt peak ratio: 0.35)

Szilard et al. (2010): Chr15:874335-874345

Müller et al. (2010): Chr15:874175-874700 (orc1-bah-delta/wt peak ratio: 0.94)


Studies that measured the replication time of this origin

Raghuraman et al. (2001): Chr15:872260 (Trep: 19.4 min.) (Confidence: 5)

Yabuki et al. (2002): Chr15:873721 (Trep: 23.3 min.)

Alvino et al. (2007): Chr15:873890 (Peak first observed at 12.5 min.)


Studies that measured the activity of this origin in hydroxyurea (HU)

Feng et al. (2006): Chr15:874000 (Activity detected in: rad53)

Crabbé et al. (2010): Chr15:874190-874434 (Activity detected in: wild-type, rad9, rev3 rad30, ctf4, ddc1, rad24, pol2-12, elg1, tof1, mrc1-AQ, mrc1-AQ rad9, ctf8, ctf18, mrc1, dcc1, ctf18 rad9, mec1-100, rad53, mec1)


Studies that predicted the location of this origin

This origin has not been predicted by any curated study.



ARS at XV-874 has unique ID: 578

Studies that confirmed an essential ACS element

  Nieduszynski et al. (2006):        ATTTTAATATTTGTT                   

Studies that predicted an essential ACS element

  Eaton et al. (2010):      ACATTTTAATATTTGTTAGGATTCGCCTTTATT   
ACS LOGO:   ACS_logo  

ARS at XV-874 has unique ID: 578

Alignments from the UCSC genome browser

Confirmed ACS
*

ARS at XV-874 has unique ID: 578

These notes are manually curated. To submit notes for this replication origin site please contact us.

There are no notes entered for this replication origin site.

ARS at XV-874 has unique ID: 578

Genome-wide studies that identified this origin

Raghuraman et al. (2001): PubMed | Science

Wyrick et al. (2001): PubMed | Science

Yabuki et al. (2002): PubMed

Feng et al. (2006): PubMed | PubMed Central | Nat. Cell Biol.

Xu et al. (2006): PubMed | PubMed Central | BMC Genomics

Alvino et al. (2007): PubMed | PubMed Central | Mol. Cell. Biol.

Shor et al. (2009): PubMed | PubMed Central | PLoS Genet.

Szilard et al. (2010): PubMed | PubMed Central | Nat. Struct. Mol. Biol.

Müller et al. (2010): PubMed | PubMed Central | Genes Dev.

Crabbé et al. (2010): PubMed | Nat. Struct. Mol. Biol.


Studies that cloned this origin

Nieduszynski et al. (2006): PubMed | PubMed Central | Genes Dev.


Studies that analyzed this origin by 2D gel

None identified.


Studies that confirmed an essential ACS element

Nieduszynski et al. (2006): PubMed | PubMed Central | Genes Dev.


Studies that predicted an essential ACS element

Eaton et al. (2010): PubMed | PubMed Central | Genes Dev.


ARS at XV-874 has unique ID: 578