Confirmed ARS at XIII-468

ARS1316

Other names: proARS1316

Status: Confirmed ARS: Confirmed by ARS assay and identified by 8 genome-wide studies.

Genomic Location: Chr13:468177-468468
Within convergent intergenic space between YMR100W and YMR101C.
View at UCSC genome browser
View on SGD Chromosome Features Map
View at SGD gbrowser site
View at Ensembl browser

DNA Sequence:

Origin Sequence Elements: ACS predicted.

Helical Stability: Minimum -148.4 ΔG° (kcal/mol) at location 468407.

Time of Origin Replication (Trep): Raghuraman et al. (2001) — Trep: 27.2 min.
Yabuki et al. (2002) — Trep: 31.8 min.
Alvino et al. (2007) — Peak first observed at 12.5 min.

Origin activity in HU: Origin activity detected in HU — more details.

ARS at XIII-468 has unique ID: 471

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ARS at XIII-468 has unique ID: 471

Studies that cloned this origin

Nieduszynski et al. (2006): Chr13:468177-468468


Studies that analyzed this origin by 2D gel

None curated.


Studies that detected this origin by chromatin immunoprecipitation (ChIP)

Wyrick et al. (2001): Chr13:468161-468444

Xu et al. (2006): Chr13:467825-469235

Szilard et al. (2010): Chr13:468455-468465


Studies that measured the replication time of this origin

Raghuraman et al. (2001): Chr13:470314 (Trep: 27.2 min.) (Confidence: 7)

Yabuki et al. (2002): Chr13:470074 (Trep: 31.8 min.)

Alvino et al. (2007): Chr13:468750 (Peak first observed at 12.5 min.)


Studies that measured the activity of this origin in hydroxyurea (HU)

Feng et al. (2006): Chr13:467000 (Activity detected in: rad53)

Crabbé et al. (2010): Chr13:468177-468468 (Activity detected in: mrc1-AQ, mrc1-AQ rad9, ctf8, ctf18, mrc1, dcc1, ctf18 rad9, mec1-100, rad53, mec1)


Studies that predicted the location of this origin

This origin has not been predicted by any curated study.



ARS at XIII-468 has unique ID: 471

Studies that confirmed an essential ACS element

None curated.


Studies that predicted an essential ACS element

  Nieduszynski et al. (2006):       TTTTTTTATTTTTTGT                   
  Xu et al. (2006):      TTTTTTTATTTTTTGTTCCTAATTTTTTTTTTTT  
ACS LOGO:   ACS_logo  

ARS at XIII-468 has unique ID: 471

Alignments from the UCSC genome browser

Predicted ACS
S. cerevisiaeACGGAGTTTTTTGTTTTCGCTTTTTTTATTTTTTGTTCCTAATTTTTTTTTTTTTT
S. kudriavzeviiCCGGAGTTCTCAATTTTCATTTTTTCGTTTCTTTGTTCTCAATTTTTTTTA-----
S. mikataeATGGAGTTTCTT-------TTTATTTTATTTTTTGTT-------TTTCTACTTTTT
S. paradoxusACGGAGTTTTTTGTTTTCGTTTTTTTTATTTTTTGTTCCTCATTTTTTTCTTA---
S. bayanusAAGAAGTTG----------TTTTTTTCATTTTTTTTTTCAATTTTATTTTGTTATT
:*:****:**:**::**:***:**::*:*:*:

ARS at XIII-468 has unique ID: 471

These notes are manually curated. To submit notes for this replication origin site please contact us.

There are no notes entered for this replication origin site.

ARS at XIII-468 has unique ID: 471

Genome-wide studies that identified this origin

Raghuraman et al. (2001): PubMed | Science

Wyrick et al. (2001): PubMed | Science

Yabuki et al. (2002): PubMed

Feng et al. (2006): PubMed | PubMed Central | Nat. Cell Biol.

Xu et al. (2006): PubMed | PubMed Central | BMC Genomics

Alvino et al. (2007): PubMed | PubMed Central | Mol. Cell. Biol.

Szilard et al. (2010): PubMed | PubMed Central | Nat. Struct. Mol. Biol.

Crabbé et al. (2010): PubMed | Nat. Struct. Mol. Biol.


Studies that cloned this origin

Nieduszynski et al. (2006): PubMed | PubMed Central | Genes Dev.


Studies that analyzed this origin by 2D gel

None identified.


Studies that confirmed an essential ACS element

None identified.


Studies that predicted an essential ACS element

Nieduszynski et al. (2006): PubMed | PubMed Central | Genes Dev.

Xu et al. (2006): PubMed | PubMed Central | BMC Genomics


ARS at XIII-468 has unique ID: 471